Experimental and statistical post-validation of positive example EST sequences carrying peroxisome targeting signals type 1 (PTS1)
نویسندگان
چکیده
منابع مشابه
Hsp70 regulates the interaction between the peroxisome targeting signal type 1 (PTS1)-receptor Pex5p and PTS1.
The peroxisome targeting signal type 1 (PTS1) receptor, Pex5p, of the tetratricopeptide repeat (TPR) motif family is located mostly in the cytosol and mediates the translocation of PTS1 proteins to peroxisomes. As a step towards understanding the mechanisms of protein import into peroxisomes, we investigated the molecular mechanisms involved in PTS1 recognition by Pex5p with regard to requireme...
متن کاملIdentification of PEX5p-related novel peroxisome-targeting signal 1 (PTS1)-binding proteins in mammals.
Based on peroxin protein 5 (Pex5p) homology searches in the expressed sequence tag database and sequencing of large full-length cDNA inserts, three novel and related human cDNAs were identified. The brain-derived cDNAs coded for two related proteins that differ only slightly at their N-terminus, and exhibit 39.8% identity to human PEX5p. The shorter liver-derived cDNA coded for the C-terminal t...
متن کاملA proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Most proteins are targeted to the peroxisomal matrix by virtue of a peroxisomal targeting signal-1 (PTS1), a short carboxy-terminal sequence specifically recognized by the PTS1 receptor Pex5p. We had previously developed a model that allowed the estimation of the affinities of many PTS1 sequences within the human proteome for Pex5p that revealed a wide range of predicted affinities. We have now...
متن کاملIdentification of novel plant peroxisomal targeting signals by a combination of machine learning methods and in vivo subcellular targeting analyses.
In the postgenomic era, accurate prediction tools are essential for identification of the proteomes of cell organelles. Prediction methods have been developed for peroxisome-targeted proteins in animals and fungi but are missing specifically for plants. For development of a predictor for plant proteins carrying peroxisome targeting signals type 1 (PTS1), we assembled more than 2500 homologous p...
متن کاملSpecification of the peroxisome targeting signals type 1 and type 2 of plant peroxisomes by bioinformatics analyses.
To specify the C-terminal peroxisome targeting signal type 1 (PTS1) and the N-terminal PTS2 for higher plants, a maximum number of plant cDNAs and expressed sequence tags that are homologous to PTS1- and PTS2-targeted plant proteins was retrieved from the public databases and the primary structure of their targeting domains was analyzed for conserved properties. According to their high overall ...
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ژورنال
عنوان ژورنال: Plant Signaling & Behavior
سال: 2012
ISSN: 1559-2324
DOI: 10.4161/psb.18720